Convallatoxin Inhibits Cell Proliferation and Induces Cell Apoptosis by Attenuating the Akt-E2F1 Signaling Pathway in K562 Cells

نویسندگان

چکیده

Objective: To determine the effect of convallatoxin on K562 cell proliferation and apoptosis. Methods: CCK-8 assay was used to detect proliferation; PI staining, JC-1 Annexin V-FITC/PI double staining were analyze cycle, mitochondrial membrane potential, apoptosis; Western blotting cleaved caspase-9, caspase-3, Bcl-2, Bax, E2F1 expression Akt phosphorylation. Subsequently, AutoDock software interaction between Akt1. Results: Upon treatment with convallatoxin, cells inhibited, arrested at S G2/M phases, apoptosis significantly induced. In addition, phosphorylation decreased, whereas overexpression rescued convallatoxin-induced a molecular docking indicated that could bind Conclusion: Convallatoxin inhibited induced mitochondrial-related in by reducing Akt-E2F1 signaling pathway, indicating it is potential agent for treating leukemia.

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ژورنال

عنوان ژورنال: Natural Product Communications

سال: 2022

ISSN: ['1555-9475', '1934-578X']

DOI: https://doi.org/10.1177/1934578x221136929